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RNA synthesis, which is also called DNA transcription, is a highly selective process. Transcription by RNA polymerase II extends beyond RNA synthesis, towards a more active role in mRNA maturation, surveillance and export to the cytoplasm.

Single-strand breaks are repaired by DNA ligase using the complementary strand of the double helix as a template, with DNA ligase creating the final phosphodiester bond to fully repair the DNA.DNA ligases discriminate against substrates containing RNA strands or mismatched base pairs at positions near the ends of the nickedDNA. Bleomycin (BLM) exerts its genotoxicity by generating free radicals, whichattack C-4′ in the deoxyribose backbone of DNA, leading to opening of the ribose ring and strand breakage; it is an S-independentradiomimetic agent that causes double-strand breaks in DNA.

First strand cDNA is synthesized using random hexamer primers and M-MuLV Reverse Transcriptase (RNase H). Second strand cDNA synthesis is subsequently performed using DNA Polymerase I and RNase H. The remaining overhangs are converted into blunt ends using exonuclease/polymerase activity. After adenylation of the 3′ ends of DNA fragments, NEBNext Adaptor with hairpin loop structure is ligated to prepare the samples for hybridization. Cell cycle and DNA replication are the top two pathways regulated by BET bromodomain inhibition. Cycloheximide blocks the translation of mRNA to protein.

Cat. No. Product Name CAS No. Information
GY02753

TH-588 hydrochloride

1640282-30-9

A potent and selective inhibitor of MTH1 protein with IC50 of 5 nM; shows no relevant inhibition for MTH2,NUDT5, NUDT12,NUDT14,NUDT16 and other proteinswith known nucleoside triphosphate pyrophosphatase activity; causees incorporation of oxidized dNTPs in cancer cells, leading to DNA damage, cytotoxicity and therapeutic responses in patient-derived mouse xenografts.

GY02747

TH-287 hydrochloride

1638211-05-8

A potent and selective inhibitor of MTH1 protein with IC50 of 0.8 nM; shows no relevant inhibition for MTH2,NUDT5, NUDT12,NUDT14,NUDT16 and other proteinswith known nucleoside triphosphate pyrophosphatase activity; causees incorporation of oxidized dNTPs in cancer cells, leading to DNA damage, cytotoxicity and therapeutic responses in patient-derived mouse xenografts.

GY02696

TH-287

1609960-30-6

2,4-Pyrimidinediamine, 6-(2,3-dichlorophenyl)-N4-methyl-

GY02648

SCR7

1533426-72-0

SCR7 is a specific inhibitor of nonhomologous end-joining (NHEJ), blocks Ligase IV-mediated joining by interfering with its DNA binding but not that of T4 DNA Ligase or Ligase I; inhibits NHEJ in a Ligase IV-dependent manner within cells, causes accumulation of double-strand breaks (DSBs), and activates the intrinsic apoptotic pathway; decreases cell proliferation of MCF7, A549, and HeLa with IC50 of 40, 34, and 44 uM, respectively; increases the efficacy of DSB-inducing therapeutic modalities in mouse xenografts; increases the efficiency of precise genome editing with CRISPR-Cas9 in mammalian cell lines and in mice.

GY10037

Synucleozid

502139-01-7

Synucleozid (NSC 377363) is a potent inhibitor of the SNCA mRNA that encodes α-synuclein protein and selectively targets the α-synuclein mRNA 5′ UTR at the designed site. Synucleozid decreases the amount of SNCA mRNA loaded into polysomes and thereby reduces α-synuclein protein levels. Synucleozid has the potential for the investigation of Parkinson’s disease.

GY06665

GS-441524

1191237-69-0

GS-441524 is a potent inhibitor of feline infectious peritonitis (FIP) virus with an EC50 of 0.78 μM.. GS-441524 strongly inhibits feline infectious peritonitis (FIP) virus in tissue culture and experimental cat infection studies. GS-441524 is a molecular precursor to a pharmacologically active nucleoside triphosphate molecule. These analogs act as an alternative substrate and RNA-chain terminator of viral RNA dependent RNA polymerase. GS-441524 was non-toxic in feline cells at concentrations as high as 100 uM and effectively inhibited FIPV replication in cultured CRFK cells and in naturally infected feline peritoneal macrophages at concentrations as low as 1 uM. Note: GS-441524 is an active metabolite of Remdesivir.

GY02216

COH-29

1190932-38-7

A small-molecule inhibitor of ribonucleotide reductase (RNR) that binds to RRM2, interfering with RRM1-RRM2 interactions with IC50 of 16 uM; overcomes hydroxyurea and gemcitabine resistance in cancer cells, effectively inhibits proliferation of most cell lines in the NCI 60 human cancer panel; inhibits nonhomologous end joining (NHEJ) efficiency in HCC1937 cells; reduced tumor growth in mouse xenograft models of human cancer.

GY01917

TH-588

1609960-31-7

A potent and selective inhibitor of MTH1 protein with IC50 of 5 nM; shows no relevant inhibition for MTH2,NUDT5, NUDT12,NUDT14,NUDT16 and other proteinswith known nucleoside triphosphate pyrophosphatase activity; causees incorporation of oxidized dNTPs in cancer cells, leading to DNA damage, cytotoxicity and therapeutic responses in patient-derived mouse xenografts.

GY05248

PNR-7-02

1633660-76-0

PNR-7-02 is a specific inhibitor of human DNA Polymerase η (hPol η) with IC50 of 8 uM, exhibits 5-10-fold specificity for hPol η over other replicative Pols; binds to a site on the little finger domain and interferes with the proper orientation of template DNA to inhibit hpol η; potentiates the effects of cisplatin in tumor cells accompanied with increased γH2AX formation.

GY05226

Mithramycin A

18378-89-7

A tricyclic pentaglycosidic, antineoplastic antibiotic from Streptomyces strains that inhibits RNA and protein synthesis by adhering to DNA; binds to the minor groove of DNA at GC-rich sites, induces cancer cell apoptosis and metastasis.

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